Journal: Advanced Science

Article Title: Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

doi: 10.1002/advs.202512679

Figure Lengend Snippet: LAB BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( L. rhamnosus ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.

Article Snippet: All lactic acid bacteria (LAB) strains were obtained through ATCC ( Lacticaseibacillus rhamnosus GG (ATCC 53 103), Lacticaseibacillus paracasei (ATCC 334), Limosilactobacillus reuteri F 275 (ATCC 23 272), L. plantarum WCFS1 (ATCC BAA‐793).

Techniques: In Vitro, Labeling, Flow Cytometry, CCK-8 Assay

Journal: Advanced Science

Article Title: Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

doi: 10.1002/advs.202512679

Figure Lengend Snippet: LAB BEVs reduce severity in acute DSS‐induced colitis. Mice received BEVs (2.5E9 particles mouse −1 day −1 , oral gavage, Days 1–7). DSS (2.5%) was given in drinking water Days 0–6, then normal water Days 7–8 (washout). A) Body‐weight change relative to Day 0. B) Colon length at Day 8. C,D) Mesenteric lymph‐node CD4+ T‐cell populations/activation at endpoint: Treg (CD4+CD8−CD25+Foxp3+), Th17 (CD4+CD8−Foxp3−RORγt+), Naive (CD4+CD44−CD62L+), Effector (CD4+CD44+CD62L−), Central memory (CD4+CD44+CD62L+). E) qPCR array of human UC–associated genes in proximal colon, shown as log2 fold‐change versus sham (vehicle/PBS) colitis. F) Pearson correlation of colitis‐associated genes comparing treatment groups with naïve/healthy mice; R^2 closer to 1 indicates greater similarity to naive. G) RT‐qPCR of selected colitis genes in pooled, bulk colon RNA. Abbreviations: L. plantarum (Lp), L. paracasei (Lc), L. reuteri (Lre), L. rhamnosus (Lrh), E. coli Nissle 1917 (EcN), E. coli DH5a (DH5a). Statistics: one‐way ANOVA with Tukey post hoc; different letters p < 0.05. If two groups share at least one letter, their means are not significantly different (α = 0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant.

Article Snippet: All lactic acid bacteria (LAB) strains were obtained through ATCC ( Lacticaseibacillus rhamnosus GG (ATCC 53 103), Lacticaseibacillus paracasei (ATCC 334), Limosilactobacillus reuteri F 275 (ATCC 23 272), L. plantarum WCFS1 (ATCC BAA‐793).

Techniques: Activation Assay, Quantitative RT-PCR

Journal: ACS Omega

Article Title: Clove and Thyme Essential Oils: From Molecular Docking to Food Application—A Study of Their Preservative Properties in Buttermilk

doi: 10.1021/acsomega.4c11538

Figure Lengend Snippet: Selectivity index of clove and thyme essential oils for pathogenic bacteria over beneficial LAB strains.

Article Snippet: The Lactic Acid Bacteria (LAB) strains, including Lactobacillus delbrueckii subsp. bulgaricus (DSM 20081) and Streptococcus thermophilus (DSM 20617), were procured from the DSMZ (German Collection of Microorganisms and Cell Cultures).

Techniques: Bacteria

Journal: ACS Omega

Article Title: Clove and Thyme Essential Oils: From Molecular Docking to Food Application—A Study of Their Preservative Properties in Buttermilk

doi: 10.1021/acsomega.4c11538

Figure Lengend Snippet: Sensitivity of Reference Bacteria to Clove and Thyme Essential Oils Assessed by Well Diffusion Assay (Inhibition Zone in mm) a

Article Snippet: The Lactic Acid Bacteria (LAB) strains, including Lactobacillus delbrueckii subsp. bulgaricus (DSM 20081) and Streptococcus thermophilus (DSM 20617), were procured from the DSMZ (German Collection of Microorganisms and Cell Cultures).

Techniques: Bacteria, Diffusion-based Assay, Inhibition, Concentration Assay

Journal: ACS Omega

Article Title: Clove and Thyme Essential Oils: From Molecular Docking to Food Application—A Study of Their Preservative Properties in Buttermilk

doi: 10.1021/acsomega.4c11538

Figure Lengend Snippet: Binding Affinity and Mechanisms of Three CEO and TEO Compounds against Pathogenic and LAB Bacteria Enzymes

Article Snippet: The Lactic Acid Bacteria (LAB) strains, including Lactobacillus delbrueckii subsp. bulgaricus (DSM 20081) and Streptococcus thermophilus (DSM 20617), were procured from the DSMZ (German Collection of Microorganisms and Cell Cultures).

Techniques: Binding Assay, Bacteria, Functional Assay

Journal: The European Chemistry and Biotechnology Journal

Article Title: Detecting viable but non-culturable lactic acid bacteria following spray-drying and during storage

doi: 10.62063/ecb-20

Figure Lengend Snippet: Figure 1. Kinetics of MTS reduction by overnight fresh cultures of (a) L. rhamnosus GG; and (b) L. plantarum 299v, at different cells concentrations: inoculum 1 (▲), inoculum 2 (●), and inoculum 3 (■). Bacterial cells were incu- bated with MTS and absorbance was read at OD490 each 20 min. Each point is the mean of three replicates.

Article Snippet: Culture media and growth conditions The encapsulation by spray-drying was performed on mesophilic lactic acid bacteria (LAB) reference strains L. rhamnosus GG (ATCC 53103) and L. plantarum 299v.

Techniques: